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mouse macrophage cell line raw264 7  (ATCC)


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    ATCC mouse macrophage cell line raw264 7
    Mouse Macrophage Cell Line Raw264 7, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 24592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/raw+264+7+mouse+macrophage+cell+line/RAW+264%2E7/pm42860561-236-0-8
    Average 99 stars, based on 24592 article reviews
    mouse macrophage cell line raw264 7 - by Bioz Stars, 2026-11
    99/100 stars

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    other:

    Article Title: Targetable Biomimetic NIR-II Theranostic Nanoplatform for Highly Efficient Multimodal Imaging-Guided Photothermal Therapy of Cervical Cancer
    Article Snippet: The HeLa human cervical cancer cell line, H8 human cervical epithelial cell line, and RAW 264.7 mouse macrophage cell line were acquired from the American Type Culture Collection (ATCC) and cultivated in DMEM medium enriched with 10% FBS and 1% penicillin-streptomycin (pen-strep) at 37 °C in a humidified incubator with 5% CO 2 .

    Expressing:

    Article Title: Pomegranate-derived nanovesicles as therapeutic agents for acute pancreatitis in a murine model: anti-inflammatory, antioxidant, and endocrine-protective properties
    Article Snippet: A HITACHI HT7800 electron microscope was used [Servicio Central Soporte e Investigación Experimental (SCSIE)]. .. The RAW 264.7 mouse macrophage cell line (ATCC, ref. TIB-71) was used to assess the transcriptional changes induced by Pg NVs in the expression of inflammatory cytokines. .. To evaluate the changes in Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation following treatment with mouse plasma EVs, we employed the RAW-Blue TM cell line (InvivoGen, San Diego, CA), which is derived from RAW 264.7 cells.

    Cell Culture:

    Article Title: Discovery of anti-inflammatory compounds from the stem bark of Garcinia latissima through in vitro and in silico approaches
    Article Snippet: .. RAW 264.7 mouse macrophage cell line (ATCC TIB-71) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (v/v), penicillin (100 units/mL) and streptomycin (100 ug/mL) and incubated in an incubator at 37 °C and 5% CO 2 . ..

    Article Title: Discovery of anti-inflammatory compounds from the stem bark of Garcinia latissima through in vitro and in silico approaches.
    Article Snippet: .. RAW 264.7 mouse macrophage cell line (ATCC TIB71) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (v/v), penicillin (100 units/mL) and streptomycin (100 ug/mL) and incubated in an incubator at 37°C and 5% CO2. ..

    Modification:

    Article Title: Discovery of anti-inflammatory compounds from the stem bark of Garcinia latissima through in vitro and in silico approaches
    Article Snippet: .. RAW 264.7 mouse macrophage cell line (ATCC TIB-71) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (v/v), penicillin (100 units/mL) and streptomycin (100 ug/mL) and incubated in an incubator at 37 °C and 5% CO 2 . ..

    Article Title: Discovery of anti-inflammatory compounds from the stem bark of Garcinia latissima through in vitro and in silico approaches.
    Article Snippet: .. RAW 264.7 mouse macrophage cell line (ATCC TIB71) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (v/v), penicillin (100 units/mL) and streptomycin (100 ug/mL) and incubated in an incubator at 37°C and 5% CO2. ..

    Incubation:

    Article Title: Discovery of anti-inflammatory compounds from the stem bark of Garcinia latissima through in vitro and in silico approaches
    Article Snippet: .. RAW 264.7 mouse macrophage cell line (ATCC TIB-71) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (v/v), penicillin (100 units/mL) and streptomycin (100 ug/mL) and incubated in an incubator at 37 °C and 5% CO 2 . ..

    Article Title: Discovery of anti-inflammatory compounds from the stem bark of Garcinia latissima through in vitro and in silico approaches.
    Article Snippet: .. RAW 264.7 mouse macrophage cell line (ATCC TIB71) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (v/v), penicillin (100 units/mL) and streptomycin (100 ug/mL) and incubated in an incubator at 37°C and 5% CO2. ..



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    The plcP mutant of B. cenocepacia shows a defect in intracellular survival in <t>RAW264.7</t> macrophages. A , confocal microscopy of the wild type (MH1K) and the plcP mutant (MH1KΔ plcP ) containing mCherry expressing plasmid pJT05 in RAW264.7 macrophages at 2 and 24 hpi. Red fluorescence indicates mCherry expressing bacterial cells, and blue fluorescence indicates DAPI staining of the macrophage nucleus. TL: transmitted light microscopy image. Scale bars indicate 10 μm. Wild type bacteria replicate after 24 h intracellularly contrary to the Δ plcP mutant which did not replicate intracellularly. B , the percentage of RAW264.7 macrophages which contain at least 1 bacterial cell of the wild type ( red ) or the mutant ( blue ) expressing the mCherry plasmid, pJT05. Around 100 cells were counted in triplicate and error bars represent ± standard deviation. A significant increase in the wild type containing macrophages can be seen over time, but not for the mutant containing macrophages. ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001 (Independent t test). C , bacterial survival measured as log 10 CFU/ml recovered from RAW264.7 infected macrophages. Significant difference in CFU/ml between the wild type and the mutant at 24 hpi (∗∗, p ≤ 0.01, independent t test). Error bars represent ± standard deviation (n = 3).
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    Image Search Results


    The plcP mutant of B. cenocepacia shows a defect in intracellular survival in RAW264.7 macrophages. A , confocal microscopy of the wild type (MH1K) and the plcP mutant (MH1KΔ plcP ) containing mCherry expressing plasmid pJT05 in RAW264.7 macrophages at 2 and 24 hpi. Red fluorescence indicates mCherry expressing bacterial cells, and blue fluorescence indicates DAPI staining of the macrophage nucleus. TL: transmitted light microscopy image. Scale bars indicate 10 μm. Wild type bacteria replicate after 24 h intracellularly contrary to the Δ plcP mutant which did not replicate intracellularly. B , the percentage of RAW264.7 macrophages which contain at least 1 bacterial cell of the wild type ( red ) or the mutant ( blue ) expressing the mCherry plasmid, pJT05. Around 100 cells were counted in triplicate and error bars represent ± standard deviation. A significant increase in the wild type containing macrophages can be seen over time, but not for the mutant containing macrophages. ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001 (Independent t test). C , bacterial survival measured as log 10 CFU/ml recovered from RAW264.7 infected macrophages. Significant difference in CFU/ml between the wild type and the mutant at 24 hpi (∗∗, p ≤ 0.01, independent t test). Error bars represent ± standard deviation (n = 3).

    Journal: The Journal of Biological Chemistry

    Article Title: The phospholipase PlcP mediates membrane lipid remodeling required for the intracellular survival of Burkholderia cenocepacia

    doi: 10.1016/j.jbc.2026.113600

    Figure Lengend Snippet: The plcP mutant of B. cenocepacia shows a defect in intracellular survival in RAW264.7 macrophages. A , confocal microscopy of the wild type (MH1K) and the plcP mutant (MH1KΔ plcP ) containing mCherry expressing plasmid pJT05 in RAW264.7 macrophages at 2 and 24 hpi. Red fluorescence indicates mCherry expressing bacterial cells, and blue fluorescence indicates DAPI staining of the macrophage nucleus. TL: transmitted light microscopy image. Scale bars indicate 10 μm. Wild type bacteria replicate after 24 h intracellularly contrary to the Δ plcP mutant which did not replicate intracellularly. B , the percentage of RAW264.7 macrophages which contain at least 1 bacterial cell of the wild type ( red ) or the mutant ( blue ) expressing the mCherry plasmid, pJT05. Around 100 cells were counted in triplicate and error bars represent ± standard deviation. A significant increase in the wild type containing macrophages can be seen over time, but not for the mutant containing macrophages. ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001 (Independent t test). C , bacterial survival measured as log 10 CFU/ml recovered from RAW264.7 infected macrophages. Significant difference in CFU/ml between the wild type and the mutant at 24 hpi (∗∗, p ≤ 0.01, independent t test). Error bars represent ± standard deviation (n = 3).

    Article Snippet: Mouse macrophage RAW264.7 cell lines were obtained from ATCC and cultured with Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% FBS and L-glutamine at 37 °C, 5% CO 2 .

    Techniques: Mutagenesis, Confocal, Microscopy, Expressing, Plasmid Preparation, Fluorescence, Staining, Transmitted Light, Bacteria, Standard Deviation, Infection